Effect of rice cultivar Kitaake cps mutants on drought resistance. (A and B) Representative pictures of (A) wild-type (WT), cps2 and cps4 mutant plants after drought stress treatment (withholding of water for 6 days) and (B) WT and cps2×4 plants following drought stress treatment as well as controls (continued watering). (C) Analysis of leaf water content following drought stress treatment (n = 6, error bars show standard deviation; *P < 0.05 and **P < 0.01, calculated using unpaired two-tailed Student’s t-test). Four independent experiments were performed with similar results. (D and E) Analysis of water loss during drought stress treatment from pots with (D) WT, cps2 or cps4 plants or (E) WT and cps2×4 plants (n = 5, error bars indicate standard deviation). Asterisks represent significant differences derived from one-way ANOVA followed by Dunnett’s multiple comparisons tests to compare the different lines against WT at each time point (*P < 0.05 and **P < 0.01; colors correlated to that for each cps mutant line). Four independent experiments were performed with similar results.