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. Author manuscript; available in PMC: 2021 Mar 18.
Published in final edited form as: Nat Protoc. 2021 Jan 25;16(3):1629–1646. doi: 10.1038/s41596-020-00472-3

Fig. 2 |. FANS gating strategy for sorting of cell type nuclei populations.

Fig. 2 |

Data representation of NeuN, PU.1 and OLIG2 nuclei isolated from cortical tissue of a pediatric epilepsy patient1. (a-f) Representative FACS plots generated on the MoFlo Astrios and visualized with FlowJo V10 with percentage of parent gates for each population (steps 73 – 79). (a) DAPI positive nuclei are selected (step 73) and (b - c) doublets are excluded (74–75). (d) Gating for NeuN-positive neuronal nuclei and PU.1-positive myeloid/microglia nuclei (step 76) and (e) NeuN-positive neuronal nuclei and OLIG2-positive oligodendrocyte nuclei (step 77). (f) Gating for NeuN-negative; LHX2-positive astrocyte nuclei (step 79).