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. Author manuscript; available in PMC: 2021 Mar 18.
Published in final edited form as: Nat Protoc. 2021 Jan 25;16(3):1629–1646. doi: 10.1038/s41596-020-00472-3
Stock solutions Final conc. 5 ml cushion 10 ml cushion 20 ml cushion
1.2 M sucrose 1.17 M 4.88 ml 9.76 ml 19.52 ml
1 M Tris-HCl, pH 8.0 10 mM 50 μl 100 μl 200 μl
1 M MgCl2 3 mM 15 μl 30 μl 60 μl
100 mM DTT 1 mM 50 μl 100 μl 200 μl