Figure 3.

Nucleosome-induced IL-12 secretion is increased in spleen cells of DNase1-deficient mice. Spleen cells from wild type (WT), DNase1-deficient mice (DNase1^−/−/Trap1^m/m) and TLR2/TLR4-deficient mice were cultured in the presence/absence of different concentrations of purified nucleosomes (Nuc), the nucleosome purification buffer (gradient), LPS (TLR4 agonist) or CpG-ODN (TLR9 agonist). IL-12p40/p70 secretion was estimated by ELISA. (A) Shown is one representative experiment of seven independent experiments using four independent chromatin preparations. Means and SD of triplicates are depicted. (B) Data pooled from the seven independent experiments with seven mice/group are shown. Means and SEM of individual means are depicted. *p ≤ 0.05 (two-tailed Wilcoxon matched-pairs signed rank test). (C) Shown is one representative experiment of two independent experiments. Means and SD of triplicates are depicted. (D) Shown is one representative experiment of three independent experiments. Means and SD of triplicates are depicted.