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. 2021 Mar 17;11:6137. doi: 10.1038/s41598-021-85447-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transcriptomic analysis reveals that VUB04 has a distinct expression profile at the undifferentiated stage. (A) Unsupervised hierarchical clustering and heatmap of transcriptome data of all lines tested. (B) Principal Component Analysis of dimension 1 versus dimension 2 based on normalized transcriptome data. (C) Volcano plot based on a comparison of gene expression levels between VUB04 and the control group (VUB01, VUB02 and VUB03). Genes with |log₂ fold change|> 1 and FDR < 0.05 were marked as either significantly upregulated (red) or downregulated (blue). D) Normalized expression values for selected pluripotency markers in the control group and VUB04. FC fold change. (E) Transcription factor enrichment for the top deregulated genes in VUB04 (based on top |log₂ fold change| and FDR < 0.05) done by the Enrichr tool and based on CHEA and ENCODE databases.) (F) and (G) Enrichment profile for MAPK/ERK signalling (F) and PI3K signalling (G) performed on the ranked gene list based on a comparison of the expression levels between VUB04 and the control group. Rank 0 represents the gene with the highest-ranking score. Vertical black bars represent genes within the ranked list belonging to the given pathway. NES normalized enrichment score.