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. 2021 Mar 17;12(4):286. doi: 10.1038/s41419-021-03555-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Immunoblotting analysis of SOX2 from the MDA-MB-231 cells treated with Eer I or NMS-873. GAPDH serves as a loading control. b Immunoblotting analysis of c-MYC from Eer I and NMS-873 treated MDA-MB-231 cells. GAPDH serves as a loading control. c Immunoblotting analysis of c-MYC from the MDA-MB-231 cells with p97 silenced. β-actin serves as a loading control. d c-MYC expression correlates with that of p97 in human breast cancer tissues. Left: representative immunohistochemical staining of c-MYC and p97 in the consecutive serial sections of breast cancer tissues. c-MYC and p97 expressions were classified as low and high. Bar: upper 200 μm, lower 50 μm. Right: correlation analyses of c-MYC and SOX2 expression in breast cancer tissues (n = 98). e, f Immunoblotting analysis of SKP2 from Eer I and NMS-873 treated MDA-MB-231 cells or MDA-MB-231 cells with p97 silenced. GAPDH serves as a loading control. g SKP2 expression correlates with that of p97 in human breast cancer tissues. Left: representative immunohistochemical staining of SKP2 and p97 in the consecutive serial sections of breast cancer tissues. SKP2 and p97 expressions were classified as low or high. Bar: upper 200 μm, lower 50 μm. Right: correlation analyses of SKP2 and p97 expression in breast cancer tissues (n = 75). h Immunoblotting analysis of SOX2 and SKP2 from mammospheres and adherent MDA-MB-231 cells. β-actin serves as a loading control. i Immunoblotting analysis of SOX2 and SKP2 from mammospheres with SKP2 silenced. β-actin serves as a loading control. j Mammosphere formation of MDA-MB-231 cells with SKP2 silenced. Left: the number of spheres under each treatment. Right: representative images. Bar: upper 100 μm. Data were shown as mean + SD. ***P < 0.001.