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. 2021 Mar 17;12(4):286. doi: 10.1038/s41419-021-03555-5

Fig. 5. Loss of p97 activates UPR and increases HIF-1α expression.

Fig. 5

a Immunoblotting analysis of BIP, XBP1s, ATF4, ATF6 p90, ATF6 p50, PERK, and pPERK from the MDA-MB-231 cells treated with increasing concentrations of Eer I and NMS-873. β-actin serves as a loading control. b, c qPCR analysis of BIP and CHOP from the MDA-MB-231 cells treated with increasing concentrations of Eer I or NMS-873. d, e qPCR analysis of BIP and CHOP from the MDA-MB-231 cells treated with 2.5 μM Eer I or NMS-873 from 1.5 to 24 h. f, g qPCR and immunoblotting analysis of HIF-1α from the MDA-MB-231 cells treated with 2.5 μM Eer I or NMS-873. h, i qPCR and immunoblotting analysis of HIF-1α from the MDA-MB-231 cells with p97 depleted. β-actin serves as a loading control. Data were shown as mean + SD. *P < 0.05, **P < 0.01, and ***P < 0.001.