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. 2021 Mar 17;12(4):286. doi: 10.1038/s41419-021-03555-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Pathways affected by p97 inhibition in MDA-MB-231 cells. b Microarray analysis of the representative up and downregulated genes. c qPCR analysis of CEBPD mRNAs from the MDA-MB-231 cells treated with 2.5 μM Eer I or NMS-873. d, e Immunoblotting analysis of C/EBPδ from the MDA-MB-231 cells treated with increasing concentration of Eer I or NMS-873 and the MDA-MB-231 cells treated with 2.5 μM Eer I or NMS-873 from 1.5 to 24 h. GAPDH serves as a loading control. f Immunoblotting of C/EBPδ from the MDA-MB-231 cells with p97 silenced. GAPDH serves as a loading control. g Mammosphere formation of Eer I-treated MDA-MB-231 cells and with CEBPD silenced. Upper: the number of spheres under each treatment. Lower: representative images. Bar: 100 μm. h Immunoblotting of C/EBPδ and SOX2 from the Eer I-treated MDA-MB-231 cells with CEBPD silenced. Data were shown as mean + SD. *P < 0.05, **P < 0.01, and ***P < 0.001.