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. 2021 Mar 4;12:567001. doi: 10.3389/fphar.2021.567001

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Copyright © 2021 Dvořák, Pospíšilová, Žížalová, Capková, Muchová, Vecka, Vrzáčková, Křížová, Zelenka and Vítek.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of LR and BR on superoxide production. Superoxide production was determined by flow cytometry in live cells after overnight LR and BR treatment (5 and 25 μmol/L) from the detected slope. Rotenone (ROT) was used as a positive control of superoxide generation. Basal superoxide production (dashed lines) was detected in the cells without ROT treatment. *, p < 0.05; ns, not significant compared to ROT, n = 4 for each experiment CTRL, control cells without LR and BR treatment; BR, bilirubin; LR, lumirubin. Data expressed as the change in proportion of superoxide-producing cells upon various treatments.