Figure 3.

Increased relative abundance of CCR7 expressing T lymphocytes in BM of patients with T2D. (A) Gating strategy for identification of CCR7 and CD45RA. The numbers indicate the percentage of naïve T cells (CCR7⁺ CD45RA⁺, top right quadrant), central memory T cells (TCM, CCR7⁺ CD45RA⁻, top left quadrant), effector memory T cells (TEM, CCR7⁻ CD45RA⁻, bottom left quadrant), and terminal effector T cells (TEMRA, CCR7⁻ CD45RA⁺, bottom right quadrant) gated on the forward and side scatter of the lymphocyte populations. Reported frequencies are illustrative of a representative case for each group. (B–I) Bar graphs showing the relative frequency of CCR7⁺ CD45RA⁺ naive cells in CD4⁺ (B) CD8⁺ cells (C) CCR7⁺ CD45RA⁻ TCM cells in CD4⁺ (D) and CD8⁺ cells (E), CCR7⁻ CD45RA⁻ TEM cells in CD4⁺ (F) and CD8⁺ cells (G) CCR7⁻CD45RA⁺ TEMRA cells in CD4⁺ (H) and CD8⁺ cells (I). Values are mean ± SEM, with each point representing an individual case.