Figure 7.

Effect of abatacept on immune cells of T2D mice. (A) Gating strategy for identification of CD4⁺ and CD8⁺ cells in the BM, spleen, and peripheral blood of db/db mice given vehicle or abatacept. Reported frequencies are illustrative of a representative case for each group. (B) Relative frequency of CD4⁺ cells. (C) Relative frequency of CD8⁺ cells. (D) Relative frequency of Ki67 expressing cells within CD4⁺ cells. (E) Relative frequency of Ki67 expressing cells within CD8⁺ cells. (F) Relative frequency of CD25⁺ cells within CD4⁺ cells. (G) Relative frequency of CD25⁺ cells within CD8⁺ cells. (H) Relative frequency of CD103⁺ cells within CD4⁺ cells. (I) Relative frequency of CD103⁺ cells within CD8⁺ cells. (J) Spider graph of cytokines in the BM of the abatacept (green line) and vehicle group (orange line). (K) Spider graph of cytokines in the peripheral blood of the abatacept (green line) and vehicle group (orange line). Measurements were performed in a pool of four mice per group. (L) Network analysis of regulated cytokines. Values are mean ± SEM, with each point representing an individual case.