FIGURE 5.

Functional siRNA analysis. (A) Experimental treatment scheme. LUHMES neurons were transduced with adenoviral (AV) vectors of SNCA or GFP (control virus) and treated with or without siRNAs against CCND1 and/or CCND2. LDH release as measure for cytotoxicity was determined in control 6 days after transduction. DIV, days in vitro; DPT, days post transduction. (B–D) Quantification of cytotoxicity upon treatment with siCCND1 (B), siCCND2 (C), and siCCND1 + siCCND2 (D). Experiments in panels (B–D) included lipofectamine controls (LC), non-coding siRNA controls (NC) in parallel to the coding siRNA treatment. Data was normalized to LC in control cells. Results are presented as mean ± SEM from seven biological repeats. Statistical analysis in panels (B–D) was performed by a two-way ANOVA with Bonferroni’s correction for multiple testing and, if significant, pairwise comparisons were evaluated by an unpaired t-test. **P < 0.01, ***P < 0.001; ns, not significant.