Table 2.
Details of primers and probes for screening and confirmatory assay.
| Assay | Oligonucleotide ID | Sequence (5′-3′) | |
|---|---|---|---|
| Primers and probes screening assay (E gene assay) | E gene | E_Sarbeco_F1 E_Sarbeco_R2 E_Sarbeco_P1 |
ACAGGTACGTTAATAGTTAATAGCGT ATATTGCAGCAGTACGCACACA FAM-ACACTAGCCATCCTTACTGCGCTTCG -BHQ |
| RNaseP gene (Internal control) | RNaseP Forward | AGATTTGGACCTGCGAGCG | |
| RNaseP Reverse | GAGCGGCTGTCTCCACAAGT | ||
| RNaseP Probe | FAM-TTCTGACCTGAAGGCTCTGCGCG-BHQ | ||
| Primers and probes confirmatory assay (RdRp and ORF gene assay) | RdRp | RdRP_SARSr-F2 RdRP_SARSr-R1 RdRP_SARSr-P2 Specific for Wuhan-CoV |
GTGARATGGTCATGTGTGGCGG CARATGTTAAASACACTATTAGCATA FAM-CAGGTGGAACCTCATCAGGAGATGC-QSY |
| HKU ORF gene | HKU-ORF1b-nsp14F | TGGGGYTTTACRGGTAACCT′ | |
| HKU-ORF1b-nsp14 R | AACRCGCTTAACAAAGCACTC | ||
| HKU-ORF1b-nsp14 P | FAM-TAGTTGTGATGCWATCATGACTAG-QSY |