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. 2021 Mar 4;9:641271. doi: 10.3389/fcell.2021.641271

FIGURE 6.

FIGURE 6

Overexpressed c-Myc in B-ALL cells rescues CDK9i induced cell apoptosis. (A) Relative mRNA expression levels of c-Myc in REH cells measured by qRT-PCR. (B) Protein expression of c-Myc in B-ALL cells determined by Western blot analysis. (C) Overexpressed level of c-Myc in REH cells detected by Western blot analysis. (D) Levels of HK2 and LDHA in c-Myc-overexpressing REH cells tested by Western blot analysis after intervention with SNS-032 at 0 and 400 nM. (E) Glucose uptake of vector and c-Myc-overexpressing REH cells was analyzed by flow cytometry. (F) Statistical analysis of glucose uptake of REH cells. (G,H) MMP and total ROS of vector and c-Myc-overexpressing REH cells were analyzed by flow cytometry. (I) Quantification of intracellular lactate in vector and c-Myc-overexpressing REH cells using the lactate assay kit. (J) EdU-labeled cell cycle of c-Myc-overexpressing REH cells analyzed by flow cytometry. (K) Statistical analysis of EdU-positive REH cells. (L) Annexin V- and 7-AAD-labeled cell apoptosis of c-Myc-overexpressing REH cells was analyzed by flow cytometry. (M) Statistical analysis of cell apoptosis rates of B-ALL cells. B-ALL cells were treated with SNS-032 for 24 h. Values were shown as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ****p < 0.0001.