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T cell deficiency promoted brown adipocyte formation. (A) Representative immunofluorescence of perilipin (red) and UCP-1 (green) in adipose tissues from WT and RAG1 KO mice. (B) Representative Western blot and quantitation for UCP-1 in BAT, SAT, and VAT of WT and Rag1 KO mice. (C) qRT-PCR analysis of relative mRNA expression of Ucp1, Pparg, and Ppargc1a in AT of WT and Rag1 KO mice. *P < 0.05; **P < 0.01 vs. WT mice, n = 4 per group.
