Table 1.
Sequences of the forward and reverse primers used to quantify gene expression by real-time PCR.
| Genes1 | Forward | Reverse |
|---|---|---|
| 18S | TCC AGC TAG GAA TAA TGG AAT AGG A | CCG GCC GTC CCT CTT AAT |
| CS | AGG GAT TTC ATC TGG AAC ACA CT | CAC CGT GTA GTA CTT CAT CTC CT |
| HAD | ATC CTT GCA AAT CAC GCA GTT | AAT GGA GGC CAC CAA ATC G |
| LDH | TTA ACT TGG TCC AAC GCA ACG TCA AT | TCC ACT GGG TTT GAG ACA ATC AG |
| SLC2A1 | ACA ACA CCG GCG TCA TCA A | TTG ACA TCA GCA TGG AGT TAC G |
| SLC2A3 | TGC TCA TCT TCT TCA TAT TCA CAT | ACT TCT TTG TCA GGT TCT ATG C |
| SLC2A8 | CTG GAG GAA TAC TGG GAG GC | CAC CAC CAT CAA CTG GAC AA |
| SLC2A12 | AGA GAG TGG GGA GGT TCC C | TCA GGA CGA GCC AAG ACA |
1
18S, 18S ribosomal RNA (used as housekeeping gene); CS, citrate synthase; HAD, β-hydroxyacyl-CoA dehydrogenase; LDH, lactate dehydrogenase; SLC2A1, solute carrier family 2 member 1; SLC2A3, solute carrier family 2 member 3; SLC2A8, solute carrier family 2 member 8; SLC2A12, solute carrier family 2 member 12.