Fig. 4.

Boxplots of the concentrations of (A) hydroxychloroquine and (B) azithromycin detected in tadpoles of P. cuvieri. Different lowercase letters indicate significant differences. For the quantification of each drug, we used 54 tadpoles/group, distributed in nine samples composed by a pool of 6 animals/each. AZT: azithromycin; HCQ: hydroxychloroquine; 1: 2.5 μg/L and 2: 12.5 μg/L. For both drugs, the background fluorescence of the control samples was also determined and subtracted from the samples from the tadpoles exposed to the treatments.