Figure 4.
ITC analysis of Ca2+ binding to FP of the coronaviruses. (A) SARS-2 FP, (B) SARS-2 FP triple mutant (E4A_D5A_D15A). (C) SARS-1 FP. (D) MERS FP. The peptides were titrated with CaCl2 in the pH5 buffer. The integrated data represent the enthalpy change per mole of injectant, ΔH, in units of kcal/mol as a function of the molar ratio. The data were fitted using a one-site model (parameters shown in Table 3B). Data points and fitted data are overlaid.