Fig. 3.

Antiviral activity of Ti₃C₂T_x on the infection of selected genotypes and mutations of SARS-CoV-2. Vero E6 cells were infected with SARS-CoV-2 in the presence of Ti₃C₂T_x at different dilutions (from a stock concentration of 1.1 mg/mL) and after 48 h, viral copies in cell culture supernatant were analyzed via qRT-PCR. The four different viral genotypes selected and characterized: A) GR, B) GH, C) S and D) other. E) Vero E6 cells were infected with SARS-CoV-2 in the presence of Ti₃C₂T_x at different dilutions (from a stock concentration of 1.1 mg/mL) and after 48 h, viability of cells was assessed and percentage of viability was plotted. F) Toxicity of Ti₃C₂T_x was determined in Vero E6 cells following material exposure for 4 h. Percentage of viability was assessed via LDH assay. Statistical differences: *p < 0.05; ***p < 0.001 compared to only virus group. Three independent samples were analyzed for each sample group.