Fig. 8.
Analysis of activation on peripheral blood mononuclear cells and monocytes. Cell activation staining analysis by flow cytometry. Cells were treated with 50 µg/mL Ti3C2Tx for 24 h or left untreated (Unt). LPS (2 μg/mL) was used as a positive control for PBMCs while ConA (10 μg/mL) was used as positive control for T cells. A) PBMCs were stained with CD25-PE. Cell activation was evaluated and expressed as percentage of total cell number. Data are presented as bar graph (left panel) and histogram plot (right panel). B) PBMCs were stained with CD69-PE-Cy7. Cell activation was evaluated and expressed as percentage of total cell number. Data are presented as bar graph (left panel) and histogram plot (right panel). C) CD4 T cells were stained with CD25-PE (left panel) and CD69-PE-Cy7 (right panel), and cell activation was evaluated and expressed as percentage of total cell number. D) CD8 T cells were stained with CD25-PE (left panel) and CD69-PE-Cy7 (right panel), and cell activation was evaluated and expressed as percentage of total cell number. E) Monocytes were stained with CD25-PE (left panel) and CD69-PE-Cy7 (right panel). Cell activation was evaluated and expressed as percentage of total cell number. F) C. monocytes were stained with CD25-PE (left panel) and CD69-PE-Cy7 (right panel), and cell activation was evaluated and expressed as percentage of total cell number. G) Int. monocytes were stained with CD25-PE (left panel) and CD69-PE-Cy7 (right panel), and cell activation was evaluated and expressed as percentage of total cell number. H) N.C. monocytes were stained with CD25-PE (left panel) and CD69-PE-Cy7 (right panel), and cell activation was evaluated and expressed as percentage of total cell number. Data are presented as mean ± ST.D. of three independent samples. *P < 0.05 by one-way ANOVA Tukey’s multiple comparison test.