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. 2021 Mar 17;11:6186. doi: 10.1038/s41598-021-85482-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Isolation kits produce different total DNA concentrations but similar 16S specific sequencing depth. (A) DNA concentration measured by Qubit varied significantly by DNA isolation kit used (Kruskal–Wallis p = 0.0007), with the Blood & Tissue kit recovering significantly more and the Promega kit recovering significantly less DNA compared to others (pairwise Wilcoxon rank sum < 0.05). (B) Differences in total DNA concentration did not translate to significant differences in the number of sequence reads per sample (Kruskal–Wallis p = 0.806).