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. 2021 Mar 17;11:6186. doi: 10.1038/s41598-021-85482-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of relative abundances of genera with biologically-significant representatives. We compared relative abundances of bacteria recovered from eight genera with high biologic relevance including urinary pathogens (Escherichia, Klebsiella, Enterococcus) and commensals (Lactobacillus, Corynebacterium, Prevotella, Staphylococcus, Gardnerella). The Promega kit tended to recover fewer Gram positive Corynebacteria, Enterococci, and Staphylococci compared to other kits while the PowerSoil kit recovered more Escherichia and Klebsiella compared to other kits. In follow up experiments, we did not confirm a bias in favor of Gram negative organisms for the PowerSoil kit. Rather, the PowerSoil kit was substantially influenced by contaminants in low biomass environments (See Fig. S3 and S4).