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. 2021 Mar 17;11:6086. doi: 10.1038/s41598-021-85486-x

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression pattern, phylogenetic reconstruction, and LOGO analysis of AmCYP76AD1. (a) The leaf color phenotypes and betalain absorbance spectra of the upper and lower leaves of the red-leaf cultivar (AMR) of four-week-old A. tricolor. (b) Expression levels of core betalain biosynthesis genes in the upper and lower leaves of four-week-old AMR plants analyzed by qRT-PCR. Statistically significant differences were determined using Student’s t-test (*P < 0.01 for the upper leaves of AMR vs. the lower leaves of AMR). (c) Phylogenetic tree of CYP76AD homologues. The species, families, and accession numbers of CYP76AD homologues are available in Supplementary Table S6. (d) Proportional LOGO plots of selected amino acids identified by Brockington et al⁹ were generated based on the CYP76ADα and CYP76ADβ homologues listed in (c). Positions are numbered according to the residues of AmCYP76AD1 and AmCYP76AD6.