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. 2021 Mar 17;12:1703. doi: 10.1038/s41467-021-21882-1

Fig. 10. α-Catenin intracellular protein levels direct the YAP/TAZ response to autophagy perturbations.

Fig. 10

a Representative immunoblots of CTNNA1 in multiple cell lines: MCF10A, HEK293T, HeLa, HepG2 after exposure to ATG7/10 siRNAs. Bars represent the mean ± s.e.m. (n (from left to right) = 10/4/4/6 independent experiments; ***P < 0.001, *P < 0.05; two-tailed one sample t-test). b TEAD luciferase activity in MCF10A cells exposed to CTNNA1/3 siRNAs, followed by treatment with EBSS (6 h), as indicated. Bars represent the mean ± s.e.m. (n = 6 independent experiments; ***P < 0.001, *P < 0.05; two-tailed one sample t-test). c TEAD luciferase activity in MCF10A cells exposed to CTNNA1/3 siRNAs, followed by treatment with Trehalose (100 mM, 24 h), as indicated. Bars represent the mean ± s.e.m. (n = 9 independent experiments; ***P < 0.001, **P < 0.01, *P < 0.05; two-tailed one sample t-test). d TEAD luciferase activity in MCF10A cells initially exposed to CTNNA1/3 siRNAs for 48 h followed by double ATG7/10 KD for other 48 h, as indicated. Bars represent the mean ± s.e.m. (n = 6 independent experiments; **P < 0.01; two-tailed one sample t-test). e TEAD luciferase activity in HepG2 cells transfected with mEm-empty or wild-type mEm-CTNNA1, and after, exposed to EBSS for 6 h. Bars represent the mean ± s.e.m. (n = 4 independent experiments; **P < 0.01, *P < 0.05, ns not significant; two-tailed one sample t-test). Exact P values for asterisks: a (from left to right) 0.0017, 0.0281, 0.0459, 0.0405; b 0.0001, <0.0001, 0.0160; c 0.0056, 0.0012, 0.0332; d 0.0046; e (from left to right) 0.0065, 0.0012, 0.0112.