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. 2021 Mar 17;12:1703. doi: 10.1038/s41467-021-21882-1

Fig. 4. YAP/TAZ is activated upon autophagy induction (Trehalose, SMER28) in MCF10A cells.

Fig. 4

a Representative confocal images of YAP/TAZ and F-actin (phalloidin) immunostaining in MCF10A cells cultured at high confluency and exposed to trehalose (100 mM for 24 h). Scale bars are 10 µm. The experiment was repeated at least once with similar results. b YAP/TAZ localisation in MCF10A cells exposed to trehalose (100 mM for 24 h) with at least 100 cells analysed per condition (****P < 0.0001; chi-squared test). The experiment was repeated with similar results. c Percentage of MCF10A cells with stress fibres exposed to trehalose (100 mM for 24 h) (n = 100 cells; ****P < 0.0001; chi-squared test). The experiment was repeated with similar results. d Cell area of MCF10A cells exposed to trehalose (100 mM for 24 h). Bars represent the mean ± s.e.m. (n = 50 cells; ****P < 0.0001; two-tailed t-test). e TEAD luciferase activity of MCF10A cells treated with trehalose (100 mM for 24 h). Bars represent the mean ± s.d. (n = 3 independent experiments; *P < 0.05; two-tailed one sample t-test). f TEAD luciferase activity of MCF10A cells treated with SMER28 (50 µM, 6 h). Bars represent the mean ± s.d. (n = 3 independent experiments; *P < 0.05; two-tailed one sample t-test). g Quantification of branched and spherical cellular structures of MCF10A cells exposed to either Tat-scrambled (control) or Tat-beclin1 peptide (20 µM, 48 h), as in (i). Bars represent the mean ± s.e.m. (n = 3 independent experiments; ***P < 0.001; two-tailed t-test). h Quantification of branched and spherical cellular structures of MCF10A cells exposed to either DMSO (control) or SMER28 (50 µM) as in (j). Bars represent the mean ± s.e.m. (n = 3 independent experiments; ****P < 0.0001; two-tailed t-test). i Representative bright-field images of MCF10A cells exposed to Tat-beclin1 peptide (20 µM, 48 h) and cultured in soft 3D extracellular matrix. Tat-scrambled was used to treat the control cells. Scale bars are 200 µm. The experiment was repeated twice with similar results. j Representative bright-field images of MCF10A cells exposed to SMER28 (50 µM) and cultured in soft 3D extracellular matrix. DMSO was used to treat the control cells. Scale bars are 200 µm. The experiment was repeated twice with similar results. Exact P values for asterisks: e 0.0421; f 0.0113; and g 0.0006.