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. 2021 Mar 17;12:1703. doi: 10.1038/s41467-021-21882-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Scatterplot of all proteins uniquely identified in both SILAC experiments (L light, M medium, H heavy amino acids). Fold change (x-axis and y-axis for experiment A and B, respectively) are shown as log2. Scatterplot of the proteins upregulated ≥ 2 fold under bafilomycin A1 (BafA1) treatment is shown on the right. b Representative α-catenins (CTNNA3 and CTNNA1) immunoblotting in double ATG7/10 knockdown MCF10A cells. The experiment was repeated at least twice with similar results. c CTNNA3/GAPDH (left panel) and CTNNA1/GAPDH (right panel) densitometry in MCF10A cells exposed to either control or ATG7/ATG10 siRNAs. The graphs show the mean ± s.d. (CTNNA3/ GAPDH, n = 3 independent experiments) and mean ± s.e.m. (CTNNA1/ GAPDH, n = 5 independent experiments; ***P < 0.001, *P < 0.05; two-tailed one sample t-test). d Representative immunoblots of Ctnna3 levels in wild-type and Atg16L1 hypomorph (Atg16 hyp) primary cortical neurons. The graph shows the Ctnna3/Gapdh levels: mean ± s.d. (n = 3 independent experiments; *P < 0.5; two-tailed one sample t-test). e Representative α-catenins (CTNNA3 and CTNNA1) immunoblots in MCF10A cells under starvation with EBSS (6 h). The experiment was repeated at least twice with similar results. f CTNNA3/GAPDH (left panel) and CTNNA1/GAPDH (right panel) densitometry in MCF10A cells starved in EBSS for 6 h. The graphs show the mean ± s.d. (n = 3 independent experiments; **P < 0.01, *P < 0.05; two-tailed one sample t-test). g Representative α-catenins (CTNNA3 and CTNNA1) immunoblots in MCF10A cells treated with the autophagy inducer Tat-Beclin1 peptide (20 µM for 48 h). Tat-scrambled peptide was used as control. The experiment was repeated at least twice with similar results. h Representative immunoblot of mEm-CTNNA1 in MCF10A cells exposed to BafA1 (200 nM, 16 h). The MCF10A cells were transiently transfected with mEm-CTNNA1. The graph shows the mEm-CTNNA1/GAPDH levels: mean ± s.d. (n = 6 independent experiments; *P < 0.05; two-tailed one sample t-test). i Representative immunoblot of mEm-CTNNA1 in MCF10A cells exposed to autophagy inducers: SMER28 (20 µM) and EBSS for 6 h. The graph shows the mEm-CTNNA1/GAPDH levels: mean ± s.d. (n = 3 independent experiments; **P < 0.01, *P < 0.05; two-tailed one sample t-test). Exact P values for asterisks: c (from left to right) 0.0280, 0.0009; d 0.0461; f (from left to right) 0.0069, 0.0181; h 0.0301; and i (from left to right) 0.0340, 0.0036.