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. 2021 Mar 17;12:1703. doi: 10.1038/s41467-021-21882-1

Fig. 7. LIR-defective CTNNA1 mutants are not autophagy substrates.

Fig. 7

a Representative immunoblots of MCF10A cells transiently expressing wild type, L897A-F900A, Y419A-V422A or Y419A-V422A-L897A-F900A mEm-CTNNA1 constructs and exposed to BafA1 treatment (400 nM, 6 h). GFP/GAPDH densitometry is shown on the right: mean ± s.d. (n = 5 independent experiments; *P < 0.05, ns not significant; two-tailed one sample t-test). b Representative immunoblot of MCF10A cells expressing Flag-wild-type and the indicated CTNNA1 mutants upon BafA1 treatment (400 nM, 6 h). c Representative immunoblots of MCF10A cells transiently expressing wild-type or Y419A-V422A mEm-CTNNNA1 and exposed to autophagy inducers: SMER28 (20 µM) and EBSS for 6 h. The graphs show the GFP/GAPDH densitometry of one representative experiment: mean ± s.d. (n = 3 independent experiments; **P < 0.01, *P < 0.05, ns not significant; two-tailed one sample t-test). The experiment was repeated with similar results. d Representative immunoblot of MCF10A cells expressing Flag-wild-type and the indicated CTNNA1 mutants upon exposure to different concentrations of SMER28 (20 and 50 µM) for 24 h. e Representative immunoblot of MCF10A cells expressing Flag-wild-type and the indicated CTNNA1 mutants upon exposure to EBSS for 4 h. Bars represent the mean ± s.e.m. (n = 4 independent experiments; *P < 0.05, ns not significant; two-tailed one sample t-test). Exact P values for asterisks: a (from left to right) 0.0317, 0.1743, 0.4750, 0.3386; c (from left to right) 0.0496, 0.0096, 0.5166, 0.1364; e (from left to right) 0.0336, 0.9923, 0.3824, 0.4799.