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. 2021 Mar 17;12:1703. doi: 10.1038/s41467-021-21882-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Representative confocal images of YAP/TAZ endogenous immunostaining in MCF10A cells expressing either wild-type or mutant (Y419A-V422A, L897A-F900A or Y419A-V422A-L897A-F900A) mEm-CTNNA1. Scale bars are 10 µm. The experiment was repeated three times with similar results. b YAP/TAZ localisation in MCF10A cells transfected with wild-type or mEm-CTNNA1 mutants. Bars represent the mean ± s.e.m. (n = 4 independent experiments; ***P < 0.001; two-way ANOVA). c YAP/TAZ localisation in MCF10A cells transfected with wild-type or mutants of mEm-CTNNA1 upon BafA1 exposure (200 nM, 16 h). Around 100 cells were analysed per condition —*P < 0.05, ns not significant; chi-squared test. d YAP/TAZ localisation in MCF10A cells transfected with wild-type or mutant forms of mEm-CTNNA1 upon α-catenin knockdown. Bars represent the mean ± s.d. (n = 3 independent experiments; ****P < 0.0001; two-way ANOVA). e TEAD luciferase activity in MCF10A cells expressing either wild-type or mutant (Y419A-V422A) Flag-CTNNA1. Bars represent the mean ± s.d. (n = 3 independent experiments; **P < 0.01, *P < 0.05; two-tailed one sample t-test). f Representative confocal images of BrdU-positive MCF10A cells transfected with wild-type or mutant forms of mEm-CTNNA1. Scale bars are 10 µm. The experiment was repeated three times with similar results. g Percentages of BrdU-positive cells in MCF10A cells transfected with wild-type or mutant forms of mEm-CTNNA1. Bars represent the mean ± s.e.m. (n = 4 independent experiments; ***P < 0.001, **P < 0.01, *P < 0.05; two-way ANOVA). h Size of MCF10A cells expressing either wild-type or the indicated mEm-CTNNA1 mutants. n = 25 cells per condition were analysed for measuring cell area using ZEN software. Bars represent the mean ± s.e.m (**P < 0.01, *P < 0.05; two-tailed t-test). Exact P values for asterisks: b 0.0003; e (from left to right) 0.0146, 0.0022, 0.0049; g (from left to right) 0.0043, 0.0207, 0.0019; h (from left to right) 0.0153, 0.0102, 0.0010.