Fig. 5. Importin α does not bind to CST.

a–c Coomasie-stained protein SDS–PAGE gels are shown for a Importin α, b the functional CST complex, and c CTC1 1–199. d Fluorescence polarization data for CST binding to various size single-stranded telomeric repeats. e–g ITC data is shown between importin α and various CST constructs. h–j Disorder plots showing no significant disorder within CST. Values above 0.5 are deemed likely to be disordered. Iupred was utilized to model propensity for disorder⁷¹. Blue represents data from the Anchor2 algorithm, and red represents Iupred2 algorithm results.