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. 2021 Mar 17;12(4):283. doi: 10.1038/s41419-021-03577-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Confluent PCT cells were treated or not with 30 µM GC7 for 24 h. a Measurement of lactate media content related to whole-cell proteins content. b Evaluation of cell glucose consumption corresponding to the difference between measurement of the media glucose content decrease (in the presence of glucose in media) and media glucose content increase (after glucose deprivation). Dots displayed mean ± SD of three independent experiments. *p < 0.05 evaluated using Mann–Whitney (a, b) tests. c Glucose uptake measured using 2-deoxy-d-[³H]-glucose. Results displayed cpm of 2-deoxy-d-[³H]-glucose measured in cell lysate. d RT-qPCR analysis of glucose transporters from the SGLT family. Graphic displayed the scatter plot of 9 (c) or 5 (d) independent values and mean ± SD. *p < 0.05 evaluated using Mann–Whitney test.