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. 2021 Mar 17;12(4):283. doi: 10.1038/s41419-021-03577-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Confluent PCT cells were treated or not with 30 µM GC7 for 24 h and then (a) oxygen consumption (OCR) and (b) extracellular acidification (ECAR) rates were measured using Seahorse technology. Analysed was performed first without glucose and then glucose (10 mM) and 2-deoxy-glucose (2-DG, 25 mM) were sequentially added as indicated. c Confluent PCT cells were treated or not with 30 µM GC7 for 24 h and by 25 mM 2-DG the last 8 h. Graphic displayed measurement of lactate media content after 8 h related to whole-cell proteins content. d Confluent PCT cells were treated or not with 30 µM GC7 and 25 mM 2-DG for 24 h. Mortality was evaluated using live/dead cells fluorescence assay. Representative pictures for each group are displayed and show live (green, calcein-FITC) and dead (red, ethidium bromide homodimer) cells. Scale bar is indicated. Graphics displayed scatter plot of 8 (a, b), 4 (c) and 11 (d) independent values and mean ± SD. *p < 0.05 evaluated using Kruskal–Wallis.