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The ARL3 variants T31A and C118F impaired the stabilities of the encoded proteins in HEK293T cells. HEK293T cells were transfected with empty vector, wild-type, ARL3 T31A and C118F vectors for 24 h and then treated with 100 μg/ml cycloheximide (CHX) for 1, 3, and 6 h, respectively. ARL3 protein levels were detected using the flag antibody. The protein levels of β-actin were used as endogenous control (n = 1).
