Figure 7.

Clever-1 KO macrophages do not contribute to the impaired transmigration of dermal DCs. (A) Schematic presentation of the study design illustrating the generation of bone marrow chimaeras and subsequent sensitization with OXA followed by i) challenging the ears with OXA or ii) without previous sensitization. (B) Ear thickness of CD11c⁺-YFP⁺ → WT and KO chimaeras 20 h after the OXA challenge (model i). (C–E) Whole-mount images of ear dermis (C), and quantification of the total number (D), and the percentage of CD11c⁺-YFP⁺ cells inside the lumen of ear lymphatics (E) of CD11c⁺-YFP⁺→WT and KO chimaeras 20 h after topical application of OXA (model ii). (F, G) Confocal images of auricular draining LNs (F), and quantifications of the number of CD11c⁺-YFP⁺cells in the auricular LNs (G) of CD11c⁺-YFP⁺→WT and KO chimaeras 20 h after topical application of OXA. Each data point represents one mouse. Data are expressed as mean ± SEM. **P < 0.01, ***P < 0.001, and ****P < 0.0001, two-tailed Student’s t-test. Scale bars 100 µm.