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. 2021 Mar 4;8:617789. doi: 10.3389/fvets.2021.617789

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Copyright © 2021 Orhan, Juturu, Sahin, Tuzcu, Ozercan, Durmus, Sahin and Sahin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of undenatured type II collagen (UC-II) on knee joint protein expression of metalloproteinase 3 (MMP-3; A), collagen type 1 (COL-1; B), osteoprotegerin (OPG; C), and receptor activator of NF-κB ligand (RANKL; D) levels in monosodium iodoacetate (MIA) induced osteoarthritis rats (n = 7). The densitometric analysis of the relative intensity according to the control group of the western blot bands was performed with β-actin normalization to ensure equal protein loading. Blots were repeated at least three times (n = 3) and representative blots are shown. The bars represent the mean and standard deviation of data. ANOVA and Tukey's post-hoc test were used to compare the results among different treatment groups. Statistical significance between groups is shown by: ^***P < 0.001 as compared to the control group and, ^### P < 0.001 as compared to MIA group.