Fig. 5.

Parbendazole and danazol positively affect spontaneous remyelination in a cuprizone-induced demyelination mouse model. Schematic representation of the experimental setup for cuprizone treatment and substance administration (a). Representative images of the oligodendroglial protein marker expression in corpus callosum after one week of remyelination (b-e''; g-g''). Image magnification 10x. The extent of remyelination was revealed as the percentage of PLP-expressing area in the defined region of interest between naïve, CPZ/vehicle-treated and CPZ/substance-treated groups. In a similar manner the impact of parbendazole and danazol on oligodendroglial cell differentiation and maturation was assessed as revealed by the number/mm² of mature (GSTπ, f’,h’; CC1, f'',h'') and early (PGFRα, f''',h''') oligodendroglial protein marker-positive cells along the same area of the corpus callosum. Naïve group n = 6 (n = 4 for the GSTπ analysis); residual groups n = 5 each. For each mouse four coronal sections were analysed (two from the medial and two from the caudal region) and the data are shown as mean values while error bars represent SEM. Significance was assessed using Tukey's range test following one-way ANOVA. Data were considered statistically significant (95% confidence interval) at *p < 0.05, **p < 0.01, ***p < 0.001. CPZ, cuprizone; veh, vehicle; Par, parbendazole; Dan, danazol; PLP, myelin proteolipid protein; GSTπ, glutathione-S-transferase Pi; CC1, adenomatous polyposis coli protein (APC) clone CC1; PDGFRα, platelet-derived growth factor receptor alpha. Scale bars: 100 µm.