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. Author manuscript; available in PMC: 2021 Mar 18.
Published in final edited form as: Cell Rep. 2021 Mar 2;34(9):108808. doi: 10.1016/j.celrep.2021.108808

Figure 4. CHK1i increases DNA replication regardless of the loss of LIN54 or FOXM1.

Figure 4.

(A) Relative EdU incorporation in Early S and Late S phase in LIN54 or FOXM1 KO cells after CHK1 inhibition. MFI of S phase populations per gates in Figure S2A were normalized to EV DMSO Early S populations. n = 3.

(B and C) Chromatin flow cytometry for chromatin-bound CDC45.

(B) CDC45 versus DAPI levels with cell cycle phases overlaid in the colors denoted. Box denotes >98th percentile of CDC45 in the sgEV-DMSO sample.

(C) MFI of CDC45 in Early and Late S phase. n = 3.

(D and E) Chromatin flow cytometry for chromatin-bound PCNA.

(D) PCNA versus DAPI levels with cell cycle phases overlaid in the colors denoted. Box denotes >98th percentile of PCNA in the sgEV-DMSO sample.

(E) MFI of PCNA in Early and Late S phase. n = 3. Mean ± SEM; ANOVA with Tukey correction; *p < 0.05; **p < 0.01; ***p < 0.001.