KEY RESOURCES TABLE
| REAGENT OR RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| pS1981 ATM | Biolegend | Cat# 651201; RRID: AB_10900815 |
| ATM | Cell Signaling Technologies | Cat# 2873T; RRID: AB_2062659 |
| MYBL2 | Millipore | Cat# MABE866 |
| pT487 MYBL2 | Abcam | Cat# Ab76009; RRID: AB_1309969 |
| CDC45 | Cell Signaling Technologies | Cat# 11881S; RRID: AB_2715569 |
| CDK1 | Santa Cruz Biotechnology | Cat# sc-54; RRID: AB_627224 |
| pT161 CDK1 | Cell Signaling Technologies | Cat# 9114S; RRID: AB_2074652 |
| pY15 CDK1/2 | Cell Signaling Technologies | Cat# 9111S; RRID: AB_331460 |
| CHK1 | Santa Cruz Biotechnology | Cat# sc-8408; RRID: AB_627257 |
| pS317 CHK1 | Cell Signaling Technologies | Cat# 2344S; RRID: AB_331488 |
| pS345 CHK1 | Cell Signaling Technologies | Cat# 2341S; RRID: AB_330023 |
| Cyclin E (CCNE) | Santa Cruz Biotechnology | Cat# sc-247; RRID: AB_627357 |
| Cyclin B1 | Santa Cruz Biotechnology | Cat # sc-752; RRID: AB_2072134 |
| pS2056 DNA-PKcs | Cell Signaling Technologies | Cat# 68716S |
| DNA-PKcs | Cell Signaling Technologies | Cat# 38168S; RRID: AB_2799128 |
| FOXM1 | Santa Cruz Biotechnology | Cat# sc-520;RRID: AB_631523 |
| pT600 FOXM1 | Cell Signaling Technologies | Cat# 14655S; RRID: AB_2798557 |
| GAPDH | Santa Cruz Biotechnology | Cat# sc-47724; RRID: AB_627678 |
| γ-H2AX | Millipore | Cat# 05–636-I; RRID: AB_2755003 |
| pS10 histone H3 | Cell Signaling Technologies | Cat# 3377T; RRID: AB_1549592 |
| pS10 histone H3 - Alexa 488 conjugate | Cell Signaling Technologies | Cat# 3465S; RRID: AB_10695860 |
| KAP1 | Bethyl Laboratories | Cat# A300–274A-M; RRID: AB_185559 |
| pS824 KAP1 | Bethyl Laboratories | Cat# A300–767A-M; RRID: AB_669740 |
| LIN54 | Bethyl Laboratories | Cat# A300–799A-M; RRID: AB_11218173 |
| PCNA | Cell Signaling Technologies | Cat# 2586S; RRID: AB_2160343 |
| RPA32 | Bethyl Laboratories | Cat# A300–244A-M; RRID: AB_185548 |
| pS4/S8 RPA32 | Bethyl Laboratories | Cat# A300–245A-M; RRID: AB_210547 |
| pS33 RPA32 | Bethyl Laboratories | Cat# A300–246A-M; RRID: AB_210547 |
| VINCULIN | Sigma-Aldrich | Cat# V9264; RRID: AB_10603627 |
| anti-BrdU-APC conjugate | Biolegend | Cat# 339808; RRID: AB_10895898 |
| anti-mouse IgG - HRP | Bethyl Laboratories | Cat# A90–116P; RRID: AB_67183 |
| anti-rabbit IgG - HRP | Bethyl Laboratories | Cat# A120–101P; RRID: AB_67264 |
| anti-mouse IgG - Alexa 488 conjugate | Cell Signaling Technologies | Cat# 4408S; RRID: AB_10694704 |
| Rabbit IgG isotype control | Cell Signaling Technologies | Cat# 4340S; RRID: AB_561545 |
| anti-Rabbit IgG - Alexa 488 conjugate | Life Technologies | Cat# A11008; RRID: AB_143165 |
| anti-Rabbit IgG - PE conjugate | Cell Signaling Technologies | Cat# 8885S; RRID: AB_2797677 |
| anti-Rabbit IgG - Alexa 647 conjugate | Life Technologies | Cat# A21245; RRID: AB_141775 |
| Biological samples | ||
| Brunello human genome-wide lentiviral sgRNA pooled library | Broad Institute (Doench et al., 2016) | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Prexasertib (LY2606368, CHK1i) | Eli Lilly & Co. | S7178 |
| AZ20 (ATRi-2) | Santa Cruz Biotechnology | sc-503186 |
| VE-822 (ATRi-1) | MedChem Express | HY-13902 |
| KU-55933 (ATMi) | Selleck | S1092 |
| Nedisertib (DNA-PKi) | MedChem Express | HY-10570 |
| RO-3306 (CDK1i) | Santa Cruz Biotechnology | sc-358700 |
| Adavosertib (AZD1775, WEE1i) | Selleck | S1525 |
| Doxorubicin (DOXO) | Cell Signaling Technologies | 5927S |
| Countbright Beads | Life Technologies | C36950 |
| AFDye 546 Azide | Click Chemistry Tools | 1283 |
| Alexa 647 Azide | Life Technologies | A10277 |
| CalFluor 647 Azide | Click Chemistry Tools | 1372 |
| 5-ethynyl-2′-deoxyuridine (EdU) | Life Technologies | A10044 |
| 5-bromo-2′-deoxyuridine (BrdU) | Sigma-Aldrich | B5002 |
| 4′,6-diamidino-2-phenylindole (DAPI) | Invitrogen | D3571 |
| Lipofectamine RNAiMAX | Invitrogen | 13778030 |
| DNaseI | Sigma Aldrich | D4513–1VL |
| Protease Cocktail Set I | Millipore | 539131 |
| Phosphatase Cocktail Set I | Millipore | 524624 |
| Critical commercial assays | ||
| FITC Annexin V Apoptosis Detection Kit II | BD Biosciences | 556570 |
| Deposited data | ||
| RNA-seq Data | This Paper | GEO: GSE154545 |
| Experimental models: cell lines | ||
| A549 | ATCC | CCL-185 |
| A549-Cas9 (pXPR_311) | Broad Institute | N/A |
| NCI-H460 | ATCC | HTB-177 |
| NCI-H460-Cas9 (pXPR_311) | Broad Institute | N/A |
| NCI-H23 | ATCC | CRL-5800 |
| NCI-H1299 | ATCC | CRL-5803 |
| Oligonucleotides: siRNA and other | ||
| siCTRL | Dharmacon | D-001810–10 |
| siLIN54 | Dharmacon | L-019325–01 |
| siCCNA2 | Dharmacon | L-003205–00 |
| siMYBL2 | Dharmacon | L-010444–01 |
| sgRNA and qPCR primers | This Paper | Table S3 |