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. 2021 Mar 4;11:621591. doi: 10.3389/fonc.2021.621591

Figure 3.

Figure 3

The density of skin infiltrating lymphocytes in the epidermis and skin biopsy by multiplex immunofluorescence (mIF) in patient 1 with Stevens-Johnson syndrome/toxic epidermal necrolysis SJS/TEN and patient 2 interface dermatitis toxicity (lesion 1) and bullous toxicity (lesion 2). (A) Skin infiltrating lymphocytes were absent in the epidermis in patient 1 with SJS/TEN. In patient 2, the interface dermatitis toxicity (lesion 1) and the bullous toxicity (lesion 2) exhibited CD3+ T-cells with variable density of CD8+ T-cells and Tregs and Th2 positive cells. (B) Examination of the total density of skin infiltrating lymphocytes by mIF analysis revealed that all three (SJS/TEN, interface dermatitis toxicity, and bullous toxicity) lesions analyzed consisted of CD3+ T-cells with a subset of CD8+ T-cells. All three lesions exhibited similar density of FoxP3+ cells and a minor population of TBet+ cells. The lesions from patient 2 exhibited higher density of RORγT+ cells compared to SJS/TEN lesion from patient 1.