Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 4;11:621591. doi: 10.3389/fonc.2021.621591

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Viscuse, Marques-Piubelli, Heberton, Parra, Shah, Siefker-Radtke, Gao, Goswami, Ivan, Curry and Campbell

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Clinicopathologic illustration of Patient 2. (A) Crusted thin red papules on the chest. (B) Patient 2 skin biopsy with interface dermatitis toxicity (lesion 1) with scattered dyskeratotic cells and the superficial perivascular dermal inflammatory infiltrate with eosinophils (hematoxylin and eosin, original magnification, 40x). (C, D) Immunohistochemical studies show that the inflammatory infiltrates are composed of CD4+ T-cells with a subset of CD8+ T-cells (Immunohistochemistry, anti-CD4 and anti-CD8, x400). (E, F) Multiplex immunofluorescence (mIF) studies with immune-oncology toxicity panel of anti-CD3, anti-CD8, anti-FoxP3, anti-TBet, anti-Gata3, and anti-RORγT antibodies. Patient 2, interface dermatitis toxicity (lesion 1) shows dermal inflammatory infiltrate that exhibits Th2 immunophenotype with Gata3+ cells (pink). There is a subset of Tregs, Foxp3+ cells (cyan), and Th17, RORγT positive cells (green) in the inflammatory infiltrate. (G) Erythematous patches on the abdomen. (H) Patient 2 skin biopsy with bullous toxicity (lesion 2) with scattered dyskeratotic cells (white arrows) and the superficial perivascular dermal inflammatory infiltrate with eosinophils and few neutrophils [hematoxylin and eosin (H&E), original magnification]. (I, J) Immunohistochemical studies show that the inflammatory infiltrates are composed of CD4+ T-cells with a subset of CD8+ T-cells (immunohistochemistry, anti-CD4 and anti-CD8, x400). (K, L) Multiplex immunofluorescence (mIF) studies with immune-oncology toxicity panel for patient 2; bullous toxicity (lesion 2) shows similar Th2 immunophenotype with Gata3+ cells (pink) and subsets of Tregs, Foxp3+ cells (cyan), and Th17, RORγT positive cells (green) [Vectra 3.0 spectral multiplex immunofluorescence (mIF) imaging system (PerkinElmer) and InForm 2.4.8 image analysis software Colors: Blue, DAPI; Red, CD3; Orange, CD8; Cyan, FOXP3; Yellow, TBet; Pink, GATA3; Green, RORγT].