Table 1.
Antibody and IHC information for MLH1, MSH2, MSH6, and PMS2.
| Antibody | Clone | Dilution | Source | Positive style | Antigen Retrieval* | Incubation | ||
|---|---|---|---|---|---|---|---|---|
| MLH1 | Mouse Monoclonal (M1) |
Prediluted | Ventana | Nuclear staining | 95°C, 88min | 36°C, 16min | ||
| PMS2 | Mouse Monoclonal (A16-4) |
Prediluted | Ventana | Nuclear staining | 95°C, 92min | 36°C, 6min | ||
| MSH2 | Rabbit Monoclonal (G219-1129) |
Prediluted | Ventana | Nuclear staining | 95°C, 88min | 36°C, 32min | ||
| MSH6 | Rabbit Monoclonal (SP93) |
Prediluted | Ventana | Nuclear staining | 95°, 88min | 36°C, 16min | ||
IHC, Immunohistochemistry.
*Heat-induced Antigen Retrieval by 1 mM EDTA in 10 mM Tris buffer (pH8.5).