Table 1.
Diameter (µm), centerline RBC velocity (µm/s), and wall shear rate (s−1) of individual microvessels within MVF-seeded matrices in vehicle- (control; n = 8) and EPO-treated (n = 8) C57BL/6 mice directly (d0) as well as 3, 6, 10, and 14 days after implantation, as assessed by intravital fluorescence microscopy and computer-assisted image analysis.
| 0 d | 3 d | 6 d | 10 d | 14 d | |
|---|---|---|---|---|---|
| Diameter (µm): | |||||
| Control | – | 55.7 ± 3.5 | 34.2 ± 3.0 | 21.8 ± 2.0 | 17.0 ± 1.8 |
| EPO | – | 54.2 ± 15.0 | 31.0 ± 1.9 | 14.4 ± 0.9* | 11.3 ± 1.1* |
| Centerline RBC velocity (µm/s): | |||||
| Control | – | 22.8 ± 14.0 | 152.2 ± 30.3 | 271.8 ± 46.6 | 273.4 ± 34.6 |
| EPO | 35.6 ± 16.4 | 153.6 ± 40.3 | 256.9 ± 26.3 | 300.7 ± 33.9 | |
| Wall shear rate (s−1): | |||||
| Control | – | 3.6 ± 2.4 | 36.7 ± 6.3 | 107.4 ± 20.2 | 136.6 ± 20.4 |
| EPO | – | 7.0 ± 4.0 | 42.6 ± 11.6 | 150.6 ± 23.5 | 232.7 ± 33.9 |
Mean ± SEM.
*
p < 0.05 versus control.