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. 2021 Mar 16;12:2041731421999750. doi: 10.1177/2041731421999750

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 7. — Double growth factors promoted the secretion of HMGB1 and increased the wound closure rate in HaCaT cells. (a) The supernatant was obtained from the HaCaT cells, where the HCD matrices were applied. The secreted HMGB1 protein level was detected using western blot analysis. (b) Relative band intensity was analyzed using Image J software. (c) The images demonstrating wound migration was captured at 0 and 48 h after wound scratching. The concentrations of treated recombinant proteins were 0.3 µg/mL (S-EGF) and 1 µg/mL (1:2 ratio, S-EGF+S-bFGF). (d) The wound closure percentage was measured using Image J software.

*p < 0.05. ***p < 0.001.