Histology and immunohistochemistry assay of mitochondrion-mediated apoptotic protein in vivo. Tumor slices were stained with anti-Bax, anti-Bcl, anti-Cleaved Caspase 3 antibody for immunohistochemical-assay of mitochondrion mediated apoptotic protein. Tunel staining was conducted to evaluate apoptotic cells that undergo extensive DNA degradation. Cells were treated with saline, DOX, NapFFGEE-JSK and NapFFGEE-JSK/DOX, respectively. Scale bars: 50 μm.