Fig. 4.

Reaction sensitivity of RPA coupled with electrochemical detection. The assay concentration ranged from 10³ to 10⁹ copies for 40 min under human body temperature. The variation in the peak current for each DPV curve measured during 40 min depending on different target concentrations: N gene (a) and RdRP gene (b) as well as for an NTC. DPV signals obtained at 20 min depending on template concentration: N gene (c) and RdRP gene (d). Inset shows the change in the peak current with respect to the target gene concentration.