Figure 4.
Pulmonary delivery from aerosolized LbL nano-in-micro particles. Micronized powder biodistribution in mice lungs: (A) Accumulation of the particles in mice lungs over pre-determined periods of time (λexc = 465 nm; λemm = 520 nm); (B) Region-of- interest (ROI) analysis of lungs-specific accumulation. Histological analysis of extracted lungs: (1) CHT-LbL siRNA 30 min; (2) CHT-LbL siRNA 24 h; (3) CHT 24 h; (4) Untreated of (E) unstained and (F) H&E stained lung tissue sections isolated from the mice. E was imaged with a Nikon light microscope (4x) and F was imaged using TPCM (using a 25x, N.A. 1.05 objective at 840 nm). *P < 0.05; **P < 0.01; ***P < 0.001