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. Author manuscript; available in PMC: 2021 Mar 18.
Published in final edited form as: Int J Pharm. 2020 Oct 26;591:119989. doi: 10.1016/j.ijpharm.2020.119989

Table 2.

IR-780 dye and siRNA encapsulation (EE%) and entrapment efficiencies (E%)a

IR-780
dye
initial
amount		 IR-780 dye
within LbL
nanosystems
(EE%)		 IR-780 dye
within nano-
in-micro
formulations
(E%)		 siRNA (μg)
within nano-
in-micro
formulations		 Total
amount of
siRNA in
1.5 mg of
powder (μg)		 siRNA Kras
in 1.5mg of
powder
(ug)c
0.13 mg/mL 82.2%: 0.1 mg/mL 28.7%: 0.03 mg/mL (in 71.9 mg of powder) 133.4 μg (in 71.9 mg of powder) – 28.7%b 2.8 μg 1.25 ug
a

EE% was calculated as E%. Nevertheless, the last one considers the possibility of entrapment at the surface of the microparticles.

b

Initial amount of LbL nanoparticles in the CHT mixture: 10 mg which is approximately 464.4ug of siRNA

c

CHT-LbL nanoparticles: 50% LbL siRNA Kras (with 89.3% siRNA Kras) + 50% LbL siRNA NC ⇒ % of siRNA Kras in CHT-LbL nanoparticles ≈ 44.65%