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Velocity vs [S] plot for hydrolysis of 10,11-EDT by recombinant, human sEH ([E] = 3 nM). The kinetic constants (KM and Vmax) were determined by nonlinear regression using the enzyme kinetic module of SigmaPlot 14.0 (Systat Software, Inc.). An R2 of 0.99 indicates that the reaction fits the Michaelis–Menten model well.
