Table 1. Optimized MRM Transitions, Retention Times, Method Limits of Detection and Quantitation, and Linear Ranges for EDTs and DHDTs.

analyte	Q1 (Da)	Q3 (Da)	tR (min)	LOD (pM)	LOQ (pM)	linear range (nM)
16,17-DHDT	365.5	235.0	6.44	50	100	0.125–200
13,14-DHDT	365.5	195.1	6.58	50	100	0.125–200
10,11-DHDT	365.5	154.9	6.67	10	50	0.125–200
7,8-DHDT	365.5	96.9	6.78	100	500	0.625–200
16,17-EDT	347.2	246.9	8.25	5	10	0.0125–200
13,14-EDT	347.2	194.9	8.44	5	10	0.0125–200
10,11-EDT	347.2	182.9	8.50	10	50	0.125–200
7,8-EDT	347.2	134.9	8.71	500	1000	1.25–200