Table 3. Kinetic Parameters for Hydrolysis of EDT Regioisomers by Recombinant Human sEH.
R2 | KM (μM) | kcat (s–1) | kcat/KM (s–1/μM–1) | |
---|---|---|---|---|
7,8-EDT | 0.95 | 30.2 | 0.26 | 0.009 |
10,11-EDT | 0.99 | 23.6 | 0.40 | 0.017 |
13,14-EDT | 0.97 | 6.37 | 0.58 | 0.091 |
16,17-EDT | 0.98 | 23.1 | 0.67 | 0.029 |
8,9-EETa | 0.97 | 26.0 | 0.56 | 0.022 |
11,12-EETa | 0.97 | 2.0 | 0.26 | 0.13 |
14,15-EETa | 0.95 | 7.0 | 3.0 | 0.43 |
Literature values.25