Fig. 3.

Biosensor setup and validation for the high-throughput screen. (a) The metabolic pathway for PCA and CCM biosynthesis. (b) Fluorescence output of CCM-responsive BenM biosensor in CCM producing strains. (c) Flow cytometric analysis of the CCM producing strains harboring BenM biosensor. (d) Fluorescence output of PCA-responsive PcaQ biosensor in PCA producing strains. MM, mineral medium, SD-CSM, synthetic defined medium with complete supplement mixture. Data shown in panel b and d are mean values ± SDs of biological triplicates, while a representative sample is shown for flow cytometric analysis in panel c and d.